Recombinant:Article Title: Downstream transcription promotes human recurrent CNV associated AT-rich sequence mediated genome rearrangements in yeast.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies cruciform DNA antibody GeneTex Cat#GTX54648 Chemicals, peptides, and recombinant proteins Mouse IgG (H + L) Sangon Cat#D110503 Camptothecin Acmec Cat#7689-03-4 Etoposide Beyotime Cat#SC0173 Methyl methanesulfonate Acmec Cat#66-27-3 Protein A/G beads MCE Cat#HY-K0202 G418 Sangon Cat#A600958 Canavanine sulfate salt Sangon Cat#A606173 5-fluoroorotic acid Macklin Cat#F832427 Zeocin Selection Antibiotic, Sterile MCE CatHY-K1053 Hydrogen peroxide solution Macklin Cat#H792073 DNA marker III Tiangen Cat#MD103 Zirconia beads Youlisheng Cat#111178579 Snailase Solarbio #S8280 Critical commercial assays TIANamp Genomic DNA Kit Tiangen Cat#DP304 Es Taq Master Mix CWbio Cat#CW0690 Universal DNA Purification Kit Tiangen Cat#DP214 Hieff Clone Zero TOPO-TA Simple Cloning Kit Yeasen Cat#10908ES20 DNA Purification Kit with Magnetic Beads Beyotime Cat#D0041M Rapid Taq Master Mix Vazyme Cat#P222-01 EZ-10 DNAaway RNA Mini-Preps Kit Sangon Cat#B618133 HiScript III 1st Strand cDNA Synthesis Kit Vazyme Cat#R312-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q111-02 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat#DC102-01 Deposited data Pacbio HiFi Seq data deposited on Genome Sequence Archive (https://ngdc.cncb.ac.cn/gsa) This paper CRA018885 MCF-7 RNA-seq data SRA SRR19737218 MCF-7 END_seq GEO GSE99194 clinvarCNV UCSC https://hgdownload.soe.ucsc.edu/goldenPath/ hg19/database/ Recurrent CNV data ClinGen https://search.clinicalgenome.org/kb/downloads Experimental models: Organisms/strains Yeast strains This paper Table S1 Oligonucleotides Primers This paper Table S2 Recombinant DNA 16p11.2_AT-rich and 16p11.2_Control cassettes in yeast This paper Data S1 22q11.2_AT-rich and 22q11.2_Control cassettes in yeast This paper Data S2 Software and algorithms MACS3 Zhang et al. https://github.com/macs3-project/MACS Integrative Genomics Viewer IGV team https://igv.org/ (Continued on next page) iScience 27, 111508, December 20, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Jupyter notebook Jupyter https://jupyter.org/ Flye-2.9.5 Pavel Pevzner’s lab https://github.com/mikolmogorov/Flye AT-rich sequence extration code This paper Data S4
Article Title: Prostate cancer cell-derived exosomal IL-8 fosters immune evasion by disturbing glucolipid metabolism of CD8 + T cell.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FITC anti-human IFN-g Antibody Biolegend Cat#502505 RRID: AB_315230 APC anti-mouse IFN-g Antibody Biolegend Cat#505809 RRID: AB_315403 FITC anti-mouse IFN-g Antibody Biolegend Cat#505805 RRID: AB_315399 FITC anti-human/mouse Granzyme B Antibody Biolegend Cat#515403 RRID: AB_2114575 APC anti-human/mouse Granzyme B Antibody Biolegend Cat#372203 RRID: AB_2687027 PE anti-human/mouse Granzyme B Antibody Biolegend Cat#372207 RRID: AB_2687031 PE anti-human CD69 Antibody elabscience Cat#E-AB-F1138D Biological samples Prostate cancer patients’ peripheral bloods Nanjing Medical University Affiliated Cancer Hospital N/A Prostate cancer patients’ tumor tissues Nanjing Medical University Affiliated Cancer Hospital N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum (FBS) Gibco Cat#A3160801 b-mercaptoethanol Gibco Cat#21985023 Penicillin-Streptomycin (1003), sterile MCE CatHY-K1006 RBC lysis buffer Sigma Cat#11814389001 Ficoll gradient centrifugation tubes Sigma Cat#26873-85-8 glutamine MAX Gibco Cat#35050087 Recombinant human IL-2 Thermo-Fischer Cat#200–02 Recombinant mouse IL-2 novoprotein Cat#CK24 jetPRIME transfection reagent Polypus Cat#101000027 Proteinase K Roche Cat#3115887001 DAPI Invitrogen Cat#D1306 Cell Lysis Buffer (10X) Cell Signaling Cat#9803 Phosphatase inhibitors Thermo-Fischer Cat#78420 Collagenase IV Gibco Cat#17104019 AZD-5069 MCE Cat#HY-19855 GW6471 MCE Cat#HY-15372 70 mm cell sieve Corning Cat#431751 0.22 mm PVDF filter Millipore Cat#SLGP033RB Amicon Ultra-0.5 Centrifugal Filter Units (100 kDa) Millipore Cat#UFC5100 PVDF Transfer Membrane Millipore Cat#IPVH00010 7-AAD (7-Aminoactinomycin D) Thermo-Fischer Cat# A1310 Critical commercial assays MojoSortTM Human CD8 T cell Isolation Kit Biolegend Cat#480012 MojoSortTM Mouse CD8 T cell Isolation Kit Biolegend Cat#480008 MojoSortTM Mouse CD3 T cell Isolation Kit Biolegend Cat#480024 CFSE Cell Division Tracker Kit Biolegend Cat# 423801 Annexin-V/PI staining kit BD Biosci. .. Cat#556547 Fixation/permeabilization kit BD Biosci.
Selection:Article Title: Downstream transcription promotes human recurrent CNV associated AT-rich sequence mediated genome rearrangements in yeast.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies cruciform DNA antibody GeneTex Cat#GTX54648 Chemicals, peptides, and recombinant proteins Mouse IgG (H + L) Sangon Cat#D110503 Camptothecin Acmec Cat#7689-03-4 Etoposide Beyotime Cat#SC0173 Methyl methanesulfonate Acmec Cat#66-27-3 Protein A/G beads MCE Cat#HY-K0202 G418 Sangon Cat#A600958 Canavanine sulfate salt Sangon Cat#A606173 5-fluoroorotic acid Macklin Cat#F832427 Zeocin Selection Antibiotic, Sterile MCE CatHY-K1053 Hydrogen peroxide solution Macklin Cat#H792073 DNA marker III Tiangen Cat#MD103 Zirconia beads Youlisheng Cat#111178579 Snailase Solarbio #S8280 Critical commercial assays TIANamp Genomic DNA Kit Tiangen Cat#DP304 Es Taq Master Mix CWbio Cat#CW0690 Universal DNA Purification Kit Tiangen Cat#DP214 Hieff Clone Zero TOPO-TA Simple Cloning Kit Yeasen Cat#10908ES20 DNA Purification Kit with Magnetic Beads Beyotime Cat#D0041M Rapid Taq Master Mix Vazyme Cat#P222-01 EZ-10 DNAaway RNA Mini-Preps Kit Sangon Cat#B618133 HiScript III 1st Strand cDNA Synthesis Kit Vazyme Cat#R312-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q111-02 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat#DC102-01 Deposited data Pacbio HiFi Seq data deposited on Genome Sequence Archive (https://ngdc.cncb.ac.cn/gsa) This paper CRA018885 MCF-7 RNA-seq data SRA SRR19737218 MCF-7 END_seq GEO GSE99194 clinvarCNV UCSC https://hgdownload.soe.ucsc.edu/goldenPath/ hg19/database/ Recurrent CNV data ClinGen https://search.clinicalgenome.org/kb/downloads Experimental models: Organisms/strains Yeast strains This paper Table S1 Oligonucleotides Primers This paper Table S2 Recombinant DNA 16p11.2_AT-rich and 16p11.2_Control cassettes in yeast This paper Data S1 22q11.2_AT-rich and 22q11.2_Control cassettes in yeast This paper Data S2 Software and algorithms MACS3 Zhang et al. https://github.com/macs3-project/MACS Integrative Genomics Viewer IGV team https://igv.org/ (Continued on next page) iScience 27, 111508, December 20, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Jupyter notebook Jupyter https://jupyter.org/ Flye-2.9.5 Pavel Pevzner’s lab https://github.com/mikolmogorov/Flye AT-rich sequence extration code This paper Data S4
Sterility:Article Title: Downstream transcription promotes human recurrent CNV associated AT-rich sequence mediated genome rearrangements in yeast.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies cruciform DNA antibody GeneTex Cat#GTX54648 Chemicals, peptides, and recombinant proteins Mouse IgG (H + L) Sangon Cat#D110503 Camptothecin Acmec Cat#7689-03-4 Etoposide Beyotime Cat#SC0173 Methyl methanesulfonate Acmec Cat#66-27-3 Protein A/G beads MCE Cat#HY-K0202 G418 Sangon Cat#A600958 Canavanine sulfate salt Sangon Cat#A606173 5-fluoroorotic acid Macklin Cat#F832427 Zeocin Selection Antibiotic, Sterile MCE CatHY-K1053 Hydrogen peroxide solution Macklin Cat#H792073 DNA marker III Tiangen Cat#MD103 Zirconia beads Youlisheng Cat#111178579 Snailase Solarbio #S8280 Critical commercial assays TIANamp Genomic DNA Kit Tiangen Cat#DP304 Es Taq Master Mix CWbio Cat#CW0690 Universal DNA Purification Kit Tiangen Cat#DP214 Hieff Clone Zero TOPO-TA Simple Cloning Kit Yeasen Cat#10908ES20 DNA Purification Kit with Magnetic Beads Beyotime Cat#D0041M Rapid Taq Master Mix Vazyme Cat#P222-01 EZ-10 DNAaway RNA Mini-Preps Kit Sangon Cat#B618133 HiScript III 1st Strand cDNA Synthesis Kit Vazyme Cat#R312-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q111-02 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat#DC102-01 Deposited data Pacbio HiFi Seq data deposited on Genome Sequence Archive (https://ngdc.cncb.ac.cn/gsa) This paper CRA018885 MCF-7 RNA-seq data SRA SRR19737218 MCF-7 END_seq GEO GSE99194 clinvarCNV UCSC https://hgdownload.soe.ucsc.edu/goldenPath/ hg19/database/ Recurrent CNV data ClinGen https://search.clinicalgenome.org/kb/downloads Experimental models: Organisms/strains Yeast strains This paper Table S1 Oligonucleotides Primers This paper Table S2 Recombinant DNA 16p11.2_AT-rich and 16p11.2_Control cassettes in yeast This paper Data S1 22q11.2_AT-rich and 22q11.2_Control cassettes in yeast This paper Data S2 Software and algorithms MACS3 Zhang et al. https://github.com/macs3-project/MACS Integrative Genomics Viewer IGV team https://igv.org/ (Continued on next page) iScience 27, 111508, December 20, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Jupyter notebook Jupyter https://jupyter.org/ Flye-2.9.5 Pavel Pevzner’s lab https://github.com/mikolmogorov/Flye AT-rich sequence extration code This paper Data S4
Article Title: Prostate cancer cell-derived exosomal IL-8 fosters immune evasion by disturbing glucolipid metabolism of CD8 + T cell.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FITC anti-human IFN-g Antibody Biolegend Cat#502505 RRID: AB_315230 APC anti-mouse IFN-g Antibody Biolegend Cat#505809 RRID: AB_315403 FITC anti-mouse IFN-g Antibody Biolegend Cat#505805 RRID: AB_315399 FITC anti-human/mouse Granzyme B Antibody Biolegend Cat#515403 RRID: AB_2114575 APC anti-human/mouse Granzyme B Antibody Biolegend Cat#372203 RRID: AB_2687027 PE anti-human/mouse Granzyme B Antibody Biolegend Cat#372207 RRID: AB_2687031 PE anti-human CD69 Antibody elabscience Cat#E-AB-F1138D Biological samples Prostate cancer patients’ peripheral bloods Nanjing Medical University Affiliated Cancer Hospital N/A Prostate cancer patients’ tumor tissues Nanjing Medical University Affiliated Cancer Hospital N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum (FBS) Gibco Cat#A3160801 b-mercaptoethanol Gibco Cat#21985023 Penicillin-Streptomycin (1003), sterile MCE CatHY-K1006 RBC lysis buffer Sigma Cat#11814389001 Ficoll gradient centrifugation tubes Sigma Cat#26873-85-8 glutamine MAX Gibco Cat#35050087 Recombinant human IL-2 Thermo-Fischer Cat#200–02 Recombinant mouse IL-2 novoprotein Cat#CK24 jetPRIME transfection reagent Polypus Cat#101000027 Proteinase K Roche Cat#3115887001 DAPI Invitrogen Cat#D1306 Cell Lysis Buffer (10X) Cell Signaling Cat#9803 Phosphatase inhibitors Thermo-Fischer Cat#78420 Collagenase IV Gibco Cat#17104019 AZD-5069 MCE Cat#HY-19855 GW6471 MCE Cat#HY-15372 70 mm cell sieve Corning Cat#431751 0.22 mm PVDF filter Millipore Cat#SLGP033RB Amicon Ultra-0.5 Centrifugal Filter Units (100 kDa) Millipore Cat#UFC5100 PVDF Transfer Membrane Millipore Cat#IPVH00010 7-AAD (7-Aminoactinomycin D) Thermo-Fischer Cat# A1310 Critical commercial assays MojoSortTM Human CD8 T cell Isolation Kit Biolegend Cat#480012 MojoSortTM Mouse CD8 T cell Isolation Kit Biolegend Cat#480008 MojoSortTM Mouse CD3 T cell Isolation Kit Biolegend Cat#480024 CFSE Cell Division Tracker Kit Biolegend Cat# 423801 Annexin-V/PI staining kit BD Biosci. .. Cat#556547 Fixation/permeabilization kit BD Biosci.
Marker:Article Title: Downstream transcription promotes human recurrent CNV associated AT-rich sequence mediated genome rearrangements in yeast.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies cruciform DNA antibody GeneTex Cat#GTX54648 Chemicals, peptides, and recombinant proteins Mouse IgG (H + L) Sangon Cat#D110503 Camptothecin Acmec Cat#7689-03-4 Etoposide Beyotime Cat#SC0173 Methyl methanesulfonate Acmec Cat#66-27-3 Protein A/G beads MCE Cat#HY-K0202 G418 Sangon Cat#A600958 Canavanine sulfate salt Sangon Cat#A606173 5-fluoroorotic acid Macklin Cat#F832427 Zeocin Selection Antibiotic, Sterile MCE CatHY-K1053 Hydrogen peroxide solution Macklin Cat#H792073 DNA marker III Tiangen Cat#MD103 Zirconia beads Youlisheng Cat#111178579 Snailase Solarbio #S8280 Critical commercial assays TIANamp Genomic DNA Kit Tiangen Cat#DP304 Es Taq Master Mix CWbio Cat#CW0690 Universal DNA Purification Kit Tiangen Cat#DP214 Hieff Clone Zero TOPO-TA Simple Cloning Kit Yeasen Cat#10908ES20 DNA Purification Kit with Magnetic Beads Beyotime Cat#D0041M Rapid Taq Master Mix Vazyme Cat#P222-01 EZ-10 DNAaway RNA Mini-Preps Kit Sangon Cat#B618133 HiScript III 1st Strand cDNA Synthesis Kit Vazyme Cat#R312-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q111-02 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat#DC102-01 Deposited data Pacbio HiFi Seq data deposited on Genome Sequence Archive (https://ngdc.cncb.ac.cn/gsa) This paper CRA018885 MCF-7 RNA-seq data SRA SRR19737218 MCF-7 END_seq GEO GSE99194 clinvarCNV UCSC https://hgdownload.soe.ucsc.edu/goldenPath/ hg19/database/ Recurrent CNV data ClinGen https://search.clinicalgenome.org/kb/downloads Experimental models: Organisms/strains Yeast strains This paper Table S1 Oligonucleotides Primers This paper Table S2 Recombinant DNA 16p11.2_AT-rich and 16p11.2_Control cassettes in yeast This paper Data S1 22q11.2_AT-rich and 22q11.2_Control cassettes in yeast This paper Data S2 Software and algorithms MACS3 Zhang et al. https://github.com/macs3-project/MACS Integrative Genomics Viewer IGV team https://igv.org/ (Continued on next page) iScience 27, 111508, December 20, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Jupyter notebook Jupyter https://jupyter.org/ Flye-2.9.5 Pavel Pevzner’s lab https://github.com/mikolmogorov/Flye AT-rich sequence extration code This paper Data S4
DNA Purification:Article Title: Downstream transcription promotes human recurrent CNV associated AT-rich sequence mediated genome rearrangements in yeast.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies cruciform DNA antibody GeneTex Cat#GTX54648 Chemicals, peptides, and recombinant proteins Mouse IgG (H + L) Sangon Cat#D110503 Camptothecin Acmec Cat#7689-03-4 Etoposide Beyotime Cat#SC0173 Methyl methanesulfonate Acmec Cat#66-27-3 Protein A/G beads MCE Cat#HY-K0202 G418 Sangon Cat#A600958 Canavanine sulfate salt Sangon Cat#A606173 5-fluoroorotic acid Macklin Cat#F832427 Zeocin Selection Antibiotic, Sterile MCE CatHY-K1053 Hydrogen peroxide solution Macklin Cat#H792073 DNA marker III Tiangen Cat#MD103 Zirconia beads Youlisheng Cat#111178579 Snailase Solarbio #S8280 Critical commercial assays TIANamp Genomic DNA Kit Tiangen Cat#DP304 Es Taq Master Mix CWbio Cat#CW0690 Universal DNA Purification Kit Tiangen Cat#DP214 Hieff Clone Zero TOPO-TA Simple Cloning Kit Yeasen Cat#10908ES20 DNA Purification Kit with Magnetic Beads Beyotime Cat#D0041M Rapid Taq Master Mix Vazyme Cat#P222-01 EZ-10 DNAaway RNA Mini-Preps Kit Sangon Cat#B618133 HiScript III 1st Strand cDNA Synthesis Kit Vazyme Cat#R312-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q111-02 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat#DC102-01 Deposited data Pacbio HiFi Seq data deposited on Genome Sequence Archive (https://ngdc.cncb.ac.cn/gsa) This paper CRA018885 MCF-7 RNA-seq data SRA SRR19737218 MCF-7 END_seq GEO GSE99194 clinvarCNV UCSC https://hgdownload.soe.ucsc.edu/goldenPath/ hg19/database/ Recurrent CNV data ClinGen https://search.clinicalgenome.org/kb/downloads Experimental models: Organisms/strains Yeast strains This paper Table S1 Oligonucleotides Primers This paper Table S2 Recombinant DNA 16p11.2_AT-rich and 16p11.2_Control cassettes in yeast This paper Data S1 22q11.2_AT-rich and 22q11.2_Control cassettes in yeast This paper Data S2 Software and algorithms MACS3 Zhang et al. https://github.com/macs3-project/MACS Integrative Genomics Viewer IGV team https://igv.org/ (Continued on next page) iScience 27, 111508, December 20, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Jupyter notebook Jupyter https://jupyter.org/ Flye-2.9.5 Pavel Pevzner’s lab https://github.com/mikolmogorov/Flye AT-rich sequence extration code This paper Data S4
Cloning:Article Title: Downstream transcription promotes human recurrent CNV associated AT-rich sequence mediated genome rearrangements in yeast.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies cruciform DNA antibody GeneTex Cat#GTX54648 Chemicals, peptides, and recombinant proteins Mouse IgG (H + L) Sangon Cat#D110503 Camptothecin Acmec Cat#7689-03-4 Etoposide Beyotime Cat#SC0173 Methyl methanesulfonate Acmec Cat#66-27-3 Protein A/G beads MCE Cat#HY-K0202 G418 Sangon Cat#A600958 Canavanine sulfate salt Sangon Cat#A606173 5-fluoroorotic acid Macklin Cat#F832427 Zeocin Selection Antibiotic, Sterile MCE CatHY-K1053 Hydrogen peroxide solution Macklin Cat#H792073 DNA marker III Tiangen Cat#MD103 Zirconia beads Youlisheng Cat#111178579 Snailase Solarbio #S8280 Critical commercial assays TIANamp Genomic DNA Kit Tiangen Cat#DP304 Es Taq Master Mix CWbio Cat#CW0690 Universal DNA Purification Kit Tiangen Cat#DP214 Hieff Clone Zero TOPO-TA Simple Cloning Kit Yeasen Cat#10908ES20 DNA Purification Kit with Magnetic Beads Beyotime Cat#D0041M Rapid Taq Master Mix Vazyme Cat#P222-01 EZ-10 DNAaway RNA Mini-Preps Kit Sangon Cat#B618133 HiScript III 1st Strand cDNA Synthesis Kit Vazyme Cat#R312-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q111-02 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat#DC102-01 Deposited data Pacbio HiFi Seq data deposited on Genome Sequence Archive (https://ngdc.cncb.ac.cn/gsa) This paper CRA018885 MCF-7 RNA-seq data SRA SRR19737218 MCF-7 END_seq GEO GSE99194 clinvarCNV UCSC https://hgdownload.soe.ucsc.edu/goldenPath/ hg19/database/ Recurrent CNV data ClinGen https://search.clinicalgenome.org/kb/downloads Experimental models: Organisms/strains Yeast strains This paper Table S1 Oligonucleotides Primers This paper Table S2 Recombinant DNA 16p11.2_AT-rich and 16p11.2_Control cassettes in yeast This paper Data S1 22q11.2_AT-rich and 22q11.2_Control cassettes in yeast This paper Data S2 Software and algorithms MACS3 Zhang et al. https://github.com/macs3-project/MACS Integrative Genomics Viewer IGV team https://igv.org/ (Continued on next page) iScience 27, 111508, December 20, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Jupyter notebook Jupyter https://jupyter.org/ Flye-2.9.5 Pavel Pevzner’s lab https://github.com/mikolmogorov/Flye AT-rich sequence extration code This paper Data S4
Magnetic Beads:Article Title: Downstream transcription promotes human recurrent CNV associated AT-rich sequence mediated genome rearrangements in yeast.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies cruciform DNA antibody GeneTex Cat#GTX54648 Chemicals, peptides, and recombinant proteins Mouse IgG (H + L) Sangon Cat#D110503 Camptothecin Acmec Cat#7689-03-4 Etoposide Beyotime Cat#SC0173 Methyl methanesulfonate Acmec Cat#66-27-3 Protein A/G beads MCE Cat#HY-K0202 G418 Sangon Cat#A600958 Canavanine sulfate salt Sangon Cat#A606173 5-fluoroorotic acid Macklin Cat#F832427 Zeocin Selection Antibiotic, Sterile MCE CatHY-K1053 Hydrogen peroxide solution Macklin Cat#H792073 DNA marker III Tiangen Cat#MD103 Zirconia beads Youlisheng Cat#111178579 Snailase Solarbio #S8280 Critical commercial assays TIANamp Genomic DNA Kit Tiangen Cat#DP304 Es Taq Master Mix CWbio Cat#CW0690 Universal DNA Purification Kit Tiangen Cat#DP214 Hieff Clone Zero TOPO-TA Simple Cloning Kit Yeasen Cat#10908ES20 DNA Purification Kit with Magnetic Beads Beyotime Cat#D0041M Rapid Taq Master Mix Vazyme Cat#P222-01 EZ-10 DNAaway RNA Mini-Preps Kit Sangon Cat#B618133 HiScript III 1st Strand cDNA Synthesis Kit Vazyme Cat#R312-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q111-02 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat#DC102-01 Deposited data Pacbio HiFi Seq data deposited on Genome Sequence Archive (https://ngdc.cncb.ac.cn/gsa) This paper CRA018885 MCF-7 RNA-seq data SRA SRR19737218 MCF-7 END_seq GEO GSE99194 clinvarCNV UCSC https://hgdownload.soe.ucsc.edu/goldenPath/ hg19/database/ Recurrent CNV data ClinGen https://search.clinicalgenome.org/kb/downloads Experimental models: Organisms/strains Yeast strains This paper Table S1 Oligonucleotides Primers This paper Table S2 Recombinant DNA 16p11.2_AT-rich and 16p11.2_Control cassettes in yeast This paper Data S1 22q11.2_AT-rich and 22q11.2_Control cassettes in yeast This paper Data S2 Software and algorithms MACS3 Zhang et al. https://github.com/macs3-project/MACS Integrative Genomics Viewer IGV team https://igv.org/ (Continued on next page) iScience 27, 111508, December 20, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Jupyter notebook Jupyter https://jupyter.org/ Flye-2.9.5 Pavel Pevzner’s lab https://github.com/mikolmogorov/Flye AT-rich sequence extration code This paper Data S4
cDNA Synthesis:Article Title: Downstream transcription promotes human recurrent CNV associated AT-rich sequence mediated genome rearrangements in yeast.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies cruciform DNA antibody GeneTex Cat#GTX54648 Chemicals, peptides, and recombinant proteins Mouse IgG (H + L) Sangon Cat#D110503 Camptothecin Acmec Cat#7689-03-4 Etoposide Beyotime Cat#SC0173 Methyl methanesulfonate Acmec Cat#66-27-3 Protein A/G beads MCE Cat#HY-K0202 G418 Sangon Cat#A600958 Canavanine sulfate salt Sangon Cat#A606173 5-fluoroorotic acid Macklin Cat#F832427 Zeocin Selection Antibiotic, Sterile MCE CatHY-K1053 Hydrogen peroxide solution Macklin Cat#H792073 DNA marker III Tiangen Cat#MD103 Zirconia beads Youlisheng Cat#111178579 Snailase Solarbio #S8280 Critical commercial assays TIANamp Genomic DNA Kit Tiangen Cat#DP304 Es Taq Master Mix CWbio Cat#CW0690 Universal DNA Purification Kit Tiangen Cat#DP214 Hieff Clone Zero TOPO-TA Simple Cloning Kit Yeasen Cat#10908ES20 DNA Purification Kit with Magnetic Beads Beyotime Cat#D0041M Rapid Taq Master Mix Vazyme Cat#P222-01 EZ-10 DNAaway RNA Mini-Preps Kit Sangon Cat#B618133 HiScript III 1st Strand cDNA Synthesis Kit Vazyme Cat#R312-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q111-02 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat#DC102-01 Deposited data Pacbio HiFi Seq data deposited on Genome Sequence Archive (https://ngdc.cncb.ac.cn/gsa) This paper CRA018885 MCF-7 RNA-seq data SRA SRR19737218 MCF-7 END_seq GEO GSE99194 clinvarCNV UCSC https://hgdownload.soe.ucsc.edu/goldenPath/ hg19/database/ Recurrent CNV data ClinGen https://search.clinicalgenome.org/kb/downloads Experimental models: Organisms/strains Yeast strains This paper Table S1 Oligonucleotides Primers This paper Table S2 Recombinant DNA 16p11.2_AT-rich and 16p11.2_Control cassettes in yeast This paper Data S1 22q11.2_AT-rich and 22q11.2_Control cassettes in yeast This paper Data S2 Software and algorithms MACS3 Zhang et al. https://github.com/macs3-project/MACS Integrative Genomics Viewer IGV team https://igv.org/ (Continued on next page) iScience 27, 111508, December 20, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Jupyter notebook Jupyter https://jupyter.org/ Flye-2.9.5 Pavel Pevzner’s lab https://github.com/mikolmogorov/Flye AT-rich sequence extration code This paper Data S4
Real-time Polymerase Chain Reaction:Article Title: Downstream transcription promotes human recurrent CNV associated AT-rich sequence mediated genome rearrangements in yeast.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies cruciform DNA antibody GeneTex Cat#GTX54648 Chemicals, peptides, and recombinant proteins Mouse IgG (H + L) Sangon Cat#D110503 Camptothecin Acmec Cat#7689-03-4 Etoposide Beyotime Cat#SC0173 Methyl methanesulfonate Acmec Cat#66-27-3 Protein A/G beads MCE Cat#HY-K0202 G418 Sangon Cat#A600958 Canavanine sulfate salt Sangon Cat#A606173 5-fluoroorotic acid Macklin Cat#F832427 Zeocin Selection Antibiotic, Sterile MCE CatHY-K1053 Hydrogen peroxide solution Macklin Cat#H792073 DNA marker III Tiangen Cat#MD103 Zirconia beads Youlisheng Cat#111178579 Snailase Solarbio #S8280 Critical commercial assays TIANamp Genomic DNA Kit Tiangen Cat#DP304 Es Taq Master Mix CWbio Cat#CW0690 Universal DNA Purification Kit Tiangen Cat#DP214 Hieff Clone Zero TOPO-TA Simple Cloning Kit Yeasen Cat#10908ES20 DNA Purification Kit with Magnetic Beads Beyotime Cat#D0041M Rapid Taq Master Mix Vazyme Cat#P222-01 EZ-10 DNAaway RNA Mini-Preps Kit Sangon Cat#B618133 HiScript III 1st Strand cDNA Synthesis Kit Vazyme Cat#R312-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q111-02 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat#DC102-01 Deposited data Pacbio HiFi Seq data deposited on Genome Sequence Archive (https://ngdc.cncb.ac.cn/gsa) This paper CRA018885 MCF-7 RNA-seq data SRA SRR19737218 MCF-7 END_seq GEO GSE99194 clinvarCNV UCSC https://hgdownload.soe.ucsc.edu/goldenPath/ hg19/database/ Recurrent CNV data ClinGen https://search.clinicalgenome.org/kb/downloads Experimental models: Organisms/strains Yeast strains This paper Table S1 Oligonucleotides Primers This paper Table S2 Recombinant DNA 16p11.2_AT-rich and 16p11.2_Control cassettes in yeast This paper Data S1 22q11.2_AT-rich and 22q11.2_Control cassettes in yeast This paper Data S2 Software and algorithms MACS3 Zhang et al. https://github.com/macs3-project/MACS Integrative Genomics Viewer IGV team https://igv.org/ (Continued on next page) iScience 27, 111508, December 20, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Jupyter notebook Jupyter https://jupyter.org/ Flye-2.9.5 Pavel Pevzner’s lab https://github.com/mikolmogorov/Flye AT-rich sequence extration code This paper Data S4
SYBR Green Assay:Article Title: Downstream transcription promotes human recurrent CNV associated AT-rich sequence mediated genome rearrangements in yeast.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies cruciform DNA antibody GeneTex Cat#GTX54648 Chemicals, peptides, and recombinant proteins Mouse IgG (H + L) Sangon Cat#D110503 Camptothecin Acmec Cat#7689-03-4 Etoposide Beyotime Cat#SC0173 Methyl methanesulfonate Acmec Cat#66-27-3 Protein A/G beads MCE Cat#HY-K0202 G418 Sangon Cat#A600958 Canavanine sulfate salt Sangon Cat#A606173 5-fluoroorotic acid Macklin Cat#F832427 Zeocin Selection Antibiotic, Sterile MCE CatHY-K1053 Hydrogen peroxide solution Macklin Cat#H792073 DNA marker III Tiangen Cat#MD103 Zirconia beads Youlisheng Cat#111178579 Snailase Solarbio #S8280 Critical commercial assays TIANamp Genomic DNA Kit Tiangen Cat#DP304 Es Taq Master Mix CWbio Cat#CW0690 Universal DNA Purification Kit Tiangen Cat#DP214 Hieff Clone Zero TOPO-TA Simple Cloning Kit Yeasen Cat#10908ES20 DNA Purification Kit with Magnetic Beads Beyotime Cat#D0041M Rapid Taq Master Mix Vazyme Cat#P222-01 EZ-10 DNAaway RNA Mini-Preps Kit Sangon Cat#B618133 HiScript III 1st Strand cDNA Synthesis Kit Vazyme Cat#R312-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q111-02 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat#DC102-01 Deposited data Pacbio HiFi Seq data deposited on Genome Sequence Archive (https://ngdc.cncb.ac.cn/gsa) This paper CRA018885 MCF-7 RNA-seq data SRA SRR19737218 MCF-7 END_seq GEO GSE99194 clinvarCNV UCSC https://hgdownload.soe.ucsc.edu/goldenPath/ hg19/database/ Recurrent CNV data ClinGen https://search.clinicalgenome.org/kb/downloads Experimental models: Organisms/strains Yeast strains This paper Table S1 Oligonucleotides Primers This paper Table S2 Recombinant DNA 16p11.2_AT-rich and 16p11.2_Control cassettes in yeast This paper Data S1 22q11.2_AT-rich and 22q11.2_Control cassettes in yeast This paper Data S2 Software and algorithms MACS3 Zhang et al. https://github.com/macs3-project/MACS Integrative Genomics Viewer IGV team https://igv.org/ (Continued on next page) iScience 27, 111508, December 20, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Jupyter notebook Jupyter https://jupyter.org/ Flye-2.9.5 Pavel Pevzner’s lab https://github.com/mikolmogorov/Flye AT-rich sequence extration code This paper Data S4
DNA Extraction:Article Title: Downstream transcription promotes human recurrent CNV associated AT-rich sequence mediated genome rearrangements in yeast.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies cruciform DNA antibody GeneTex Cat#GTX54648 Chemicals, peptides, and recombinant proteins Mouse IgG (H + L) Sangon Cat#D110503 Camptothecin Acmec Cat#7689-03-4 Etoposide Beyotime Cat#SC0173 Methyl methanesulfonate Acmec Cat#66-27-3 Protein A/G beads MCE Cat#HY-K0202 G418 Sangon Cat#A600958 Canavanine sulfate salt Sangon Cat#A606173 5-fluoroorotic acid Macklin Cat#F832427 Zeocin Selection Antibiotic, Sterile MCE CatHY-K1053 Hydrogen peroxide solution Macklin Cat#H792073 DNA marker III Tiangen Cat#MD103 Zirconia beads Youlisheng Cat#111178579 Snailase Solarbio #S8280 Critical commercial assays TIANamp Genomic DNA Kit Tiangen Cat#DP304 Es Taq Master Mix CWbio Cat#CW0690 Universal DNA Purification Kit Tiangen Cat#DP214 Hieff Clone Zero TOPO-TA Simple Cloning Kit Yeasen Cat#10908ES20 DNA Purification Kit with Magnetic Beads Beyotime Cat#D0041M Rapid Taq Master Mix Vazyme Cat#P222-01 EZ-10 DNAaway RNA Mini-Preps Kit Sangon Cat#B618133 HiScript III 1st Strand cDNA Synthesis Kit Vazyme Cat#R312-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q111-02 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat#DC102-01 Deposited data Pacbio HiFi Seq data deposited on Genome Sequence Archive (https://ngdc.cncb.ac.cn/gsa) This paper CRA018885 MCF-7 RNA-seq data SRA SRR19737218 MCF-7 END_seq GEO GSE99194 clinvarCNV UCSC https://hgdownload.soe.ucsc.edu/goldenPath/ hg19/database/ Recurrent CNV data ClinGen https://search.clinicalgenome.org/kb/downloads Experimental models: Organisms/strains Yeast strains This paper Table S1 Oligonucleotides Primers This paper Table S2 Recombinant DNA 16p11.2_AT-rich and 16p11.2_Control cassettes in yeast This paper Data S1 22q11.2_AT-rich and 22q11.2_Control cassettes in yeast This paper Data S2 Software and algorithms MACS3 Zhang et al. https://github.com/macs3-project/MACS Integrative Genomics Viewer IGV team https://igv.org/ (Continued on next page) iScience 27, 111508, December 20, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Jupyter notebook Jupyter https://jupyter.org/ Flye-2.9.5 Pavel Pevzner’s lab https://github.com/mikolmogorov/Flye AT-rich sequence extration code This paper Data S4
Sequencing:Article Title: Downstream transcription promotes human recurrent CNV associated AT-rich sequence mediated genome rearrangements in yeast.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies cruciform DNA antibody GeneTex Cat#GTX54648 Chemicals, peptides, and recombinant proteins Mouse IgG (H + L) Sangon Cat#D110503 Camptothecin Acmec Cat#7689-03-4 Etoposide Beyotime Cat#SC0173 Methyl methanesulfonate Acmec Cat#66-27-3 Protein A/G beads MCE Cat#HY-K0202 G418 Sangon Cat#A600958 Canavanine sulfate salt Sangon Cat#A606173 5-fluoroorotic acid Macklin Cat#F832427 Zeocin Selection Antibiotic, Sterile MCE CatHY-K1053 Hydrogen peroxide solution Macklin Cat#H792073 DNA marker III Tiangen Cat#MD103 Zirconia beads Youlisheng Cat#111178579 Snailase Solarbio #S8280 Critical commercial assays TIANamp Genomic DNA Kit Tiangen Cat#DP304 Es Taq Master Mix CWbio Cat#CW0690 Universal DNA Purification Kit Tiangen Cat#DP214 Hieff Clone Zero TOPO-TA Simple Cloning Kit Yeasen Cat#10908ES20 DNA Purification Kit with Magnetic Beads Beyotime Cat#D0041M Rapid Taq Master Mix Vazyme Cat#P222-01 EZ-10 DNAaway RNA Mini-Preps Kit Sangon Cat#B618133 HiScript III 1st Strand cDNA Synthesis Kit Vazyme Cat#R312-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q111-02 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat#DC102-01 Deposited data Pacbio HiFi Seq data deposited on Genome Sequence Archive (https://ngdc.cncb.ac.cn/gsa) This paper CRA018885 MCF-7 RNA-seq data SRA SRR19737218 MCF-7 END_seq GEO GSE99194 clinvarCNV UCSC https://hgdownload.soe.ucsc.edu/goldenPath/ hg19/database/ Recurrent CNV data ClinGen https://search.clinicalgenome.org/kb/downloads Experimental models: Organisms/strains Yeast strains This paper Table S1 Oligonucleotides Primers This paper Table S2 Recombinant DNA 16p11.2_AT-rich and 16p11.2_Control cassettes in yeast This paper Data S1 22q11.2_AT-rich and 22q11.2_Control cassettes in yeast This paper Data S2 Software and algorithms MACS3 Zhang et al. https://github.com/macs3-project/MACS Integrative Genomics Viewer IGV team https://igv.org/ (Continued on next page) iScience 27, 111508, December 20, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Jupyter notebook Jupyter https://jupyter.org/ Flye-2.9.5 Pavel Pevzner’s lab https://github.com/mikolmogorov/Flye AT-rich sequence extration code This paper Data S4
RNA Sequencing:Article Title: Downstream transcription promotes human recurrent CNV associated AT-rich sequence mediated genome rearrangements in yeast.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies cruciform DNA antibody GeneTex Cat#GTX54648 Chemicals, peptides, and recombinant proteins Mouse IgG (H + L) Sangon Cat#D110503 Camptothecin Acmec Cat#7689-03-4 Etoposide Beyotime Cat#SC0173 Methyl methanesulfonate Acmec Cat#66-27-3 Protein A/G beads MCE Cat#HY-K0202 G418 Sangon Cat#A600958 Canavanine sulfate salt Sangon Cat#A606173 5-fluoroorotic acid Macklin Cat#F832427 Zeocin Selection Antibiotic, Sterile MCE CatHY-K1053 Hydrogen peroxide solution Macklin Cat#H792073 DNA marker III Tiangen Cat#MD103 Zirconia beads Youlisheng Cat#111178579 Snailase Solarbio #S8280 Critical commercial assays TIANamp Genomic DNA Kit Tiangen Cat#DP304 Es Taq Master Mix CWbio Cat#CW0690 Universal DNA Purification Kit Tiangen Cat#DP214 Hieff Clone Zero TOPO-TA Simple Cloning Kit Yeasen Cat#10908ES20 DNA Purification Kit with Magnetic Beads Beyotime Cat#D0041M Rapid Taq Master Mix Vazyme Cat#P222-01 EZ-10 DNAaway RNA Mini-Preps Kit Sangon Cat#B618133 HiScript III 1st Strand cDNA Synthesis Kit Vazyme Cat#R312-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q111-02 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat#DC102-01 Deposited data Pacbio HiFi Seq data deposited on Genome Sequence Archive (https://ngdc.cncb.ac.cn/gsa) This paper CRA018885 MCF-7 RNA-seq data SRA SRR19737218 MCF-7 END_seq GEO GSE99194 clinvarCNV UCSC https://hgdownload.soe.ucsc.edu/goldenPath/ hg19/database/ Recurrent CNV data ClinGen https://search.clinicalgenome.org/kb/downloads Experimental models: Organisms/strains Yeast strains This paper Table S1 Oligonucleotides Primers This paper Table S2 Recombinant DNA 16p11.2_AT-rich and 16p11.2_Control cassettes in yeast This paper Data S1 22q11.2_AT-rich and 22q11.2_Control cassettes in yeast This paper Data S2 Software and algorithms MACS3 Zhang et al. https://github.com/macs3-project/MACS Integrative Genomics Viewer IGV team https://igv.org/ (Continued on next page) iScience 27, 111508, December 20, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Jupyter notebook Jupyter https://jupyter.org/ Flye-2.9.5 Pavel Pevzner’s lab https://github.com/mikolmogorov/Flye AT-rich sequence extration code This paper Data S4
Software:Article Title: Downstream transcription promotes human recurrent CNV associated AT-rich sequence mediated genome rearrangements in yeast.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies cruciform DNA antibody GeneTex Cat#GTX54648 Chemicals, peptides, and recombinant proteins Mouse IgG (H + L) Sangon Cat#D110503 Camptothecin Acmec Cat#7689-03-4 Etoposide Beyotime Cat#SC0173 Methyl methanesulfonate Acmec Cat#66-27-3 Protein A/G beads MCE Cat#HY-K0202 G418 Sangon Cat#A600958 Canavanine sulfate salt Sangon Cat#A606173 5-fluoroorotic acid Macklin Cat#F832427 Zeocin Selection Antibiotic, Sterile MCE CatHY-K1053 Hydrogen peroxide solution Macklin Cat#H792073 DNA marker III Tiangen Cat#MD103 Zirconia beads Youlisheng Cat#111178579 Snailase Solarbio #S8280 Critical commercial assays TIANamp Genomic DNA Kit Tiangen Cat#DP304 Es Taq Master Mix CWbio Cat#CW0690 Universal DNA Purification Kit Tiangen Cat#DP214 Hieff Clone Zero TOPO-TA Simple Cloning Kit Yeasen Cat#10908ES20 DNA Purification Kit with Magnetic Beads Beyotime Cat#D0041M Rapid Taq Master Mix Vazyme Cat#P222-01 EZ-10 DNAaway RNA Mini-Preps Kit Sangon Cat#B618133 HiScript III 1st Strand cDNA Synthesis Kit Vazyme Cat#R312-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q111-02 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat#DC102-01 Deposited data Pacbio HiFi Seq data deposited on Genome Sequence Archive (https://ngdc.cncb.ac.cn/gsa) This paper CRA018885 MCF-7 RNA-seq data SRA SRR19737218 MCF-7 END_seq GEO GSE99194 clinvarCNV UCSC https://hgdownload.soe.ucsc.edu/goldenPath/ hg19/database/ Recurrent CNV data ClinGen https://search.clinicalgenome.org/kb/downloads Experimental models: Organisms/strains Yeast strains This paper Table S1 Oligonucleotides Primers This paper Table S2 Recombinant DNA 16p11.2_AT-rich and 16p11.2_Control cassettes in yeast This paper Data S1 22q11.2_AT-rich and 22q11.2_Control cassettes in yeast This paper Data S2 Software and algorithms MACS3 Zhang et al. https://github.com/macs3-project/MACS Integrative Genomics Viewer IGV team https://igv.org/ (Continued on next page) iScience 27, 111508, December 20, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Jupyter notebook Jupyter https://jupyter.org/ Flye-2.9.5 Pavel Pevzner’s lab https://github.com/mikolmogorov/Flye AT-rich sequence extration code This paper Data S4
Red Blood Cell Lysis:Article Title: Prostate cancer cell-derived exosomal IL-8 fosters immune evasion by disturbing glucolipid metabolism of CD8 + T cell.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FITC anti-human IFN-g Antibody Biolegend Cat#502505 RRID: AB_315230 APC anti-mouse IFN-g Antibody Biolegend Cat#505809 RRID: AB_315403 FITC anti-mouse IFN-g Antibody Biolegend Cat#505805 RRID: AB_315399 FITC anti-human/mouse Granzyme B Antibody Biolegend Cat#515403 RRID: AB_2114575 APC anti-human/mouse Granzyme B Antibody Biolegend Cat#372203 RRID: AB_2687027 PE anti-human/mouse Granzyme B Antibody Biolegend Cat#372207 RRID: AB_2687031 PE anti-human CD69 Antibody elabscience Cat#E-AB-F1138D Biological samples Prostate cancer patients’ peripheral bloods Nanjing Medical University Affiliated Cancer Hospital N/A Prostate cancer patients’ tumor tissues Nanjing Medical University Affiliated Cancer Hospital N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum (FBS) Gibco Cat#A3160801 b-mercaptoethanol Gibco Cat#21985023 Penicillin-Streptomycin (1003), sterile MCE CatHY-K1006 RBC lysis buffer Sigma Cat#11814389001 Ficoll gradient centrifugation tubes Sigma Cat#26873-85-8 glutamine MAX Gibco Cat#35050087 Recombinant human IL-2 Thermo-Fischer Cat#200–02 Recombinant mouse IL-2 novoprotein Cat#CK24 jetPRIME transfection reagent Polypus Cat#101000027 Proteinase K Roche Cat#3115887001 DAPI Invitrogen Cat#D1306 Cell Lysis Buffer (10X) Cell Signaling Cat#9803 Phosphatase inhibitors Thermo-Fischer Cat#78420 Collagenase IV Gibco Cat#17104019 AZD-5069 MCE Cat#HY-19855 GW6471 MCE Cat#HY-15372 70 mm cell sieve Corning Cat#431751 0.22 mm PVDF filter Millipore Cat#SLGP033RB Amicon Ultra-0.5 Centrifugal Filter Units (100 kDa) Millipore Cat#UFC5100 PVDF Transfer Membrane Millipore Cat#IPVH00010 7-AAD (7-Aminoactinomycin D) Thermo-Fischer Cat# A1310 Critical commercial assays MojoSortTM Human CD8 T cell Isolation Kit Biolegend Cat#480012 MojoSortTM Mouse CD8 T cell Isolation Kit Biolegend Cat#480008 MojoSortTM Mouse CD3 T cell Isolation Kit Biolegend Cat#480024 CFSE Cell Division Tracker Kit Biolegend Cat# 423801 Annexin-V/PI staining kit BD Biosci. .. Cat#556547 Fixation/permeabilization kit BD Biosci.
Gradient Centrifugation:Article Title: Prostate cancer cell-derived exosomal IL-8 fosters immune evasion by disturbing glucolipid metabolism of CD8 + T cell.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FITC anti-human IFN-g Antibody Biolegend Cat#502505 RRID: AB_315230 APC anti-mouse IFN-g Antibody Biolegend Cat#505809 RRID: AB_315403 FITC anti-mouse IFN-g Antibody Biolegend Cat#505805 RRID: AB_315399 FITC anti-human/mouse Granzyme B Antibody Biolegend Cat#515403 RRID: AB_2114575 APC anti-human/mouse Granzyme B Antibody Biolegend Cat#372203 RRID: AB_2687027 PE anti-human/mouse Granzyme B Antibody Biolegend Cat#372207 RRID: AB_2687031 PE anti-human CD69 Antibody elabscience Cat#E-AB-F1138D Biological samples Prostate cancer patients’ peripheral bloods Nanjing Medical University Affiliated Cancer Hospital N/A Prostate cancer patients’ tumor tissues Nanjing Medical University Affiliated Cancer Hospital N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum (FBS) Gibco Cat#A3160801 b-mercaptoethanol Gibco Cat#21985023 Penicillin-Streptomycin (1003), sterile MCE CatHY-K1006 RBC lysis buffer Sigma Cat#11814389001 Ficoll gradient centrifugation tubes Sigma Cat#26873-85-8 glutamine MAX Gibco Cat#35050087 Recombinant human IL-2 Thermo-Fischer Cat#200–02 Recombinant mouse IL-2 novoprotein Cat#CK24 jetPRIME transfection reagent Polypus Cat#101000027 Proteinase K Roche Cat#3115887001 DAPI Invitrogen Cat#D1306 Cell Lysis Buffer (10X) Cell Signaling Cat#9803 Phosphatase inhibitors Thermo-Fischer Cat#78420 Collagenase IV Gibco Cat#17104019 AZD-5069 MCE Cat#HY-19855 GW6471 MCE Cat#HY-15372 70 mm cell sieve Corning Cat#431751 0.22 mm PVDF filter Millipore Cat#SLGP033RB Amicon Ultra-0.5 Centrifugal Filter Units (100 kDa) Millipore Cat#UFC5100 PVDF Transfer Membrane Millipore Cat#IPVH00010 7-AAD (7-Aminoactinomycin D) Thermo-Fischer Cat# A1310 Critical commercial assays MojoSortTM Human CD8 T cell Isolation Kit Biolegend Cat#480012 MojoSortTM Mouse CD8 T cell Isolation Kit Biolegend Cat#480008 MojoSortTM Mouse CD3 T cell Isolation Kit Biolegend Cat#480024 CFSE Cell Division Tracker Kit Biolegend Cat# 423801 Annexin-V/PI staining kit BD Biosci. .. Cat#556547 Fixation/permeabilization kit BD Biosci.
Transfection:Article Title: Prostate cancer cell-derived exosomal IL-8 fosters immune evasion by disturbing glucolipid metabolism of CD8 + T cell.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FITC anti-human IFN-g Antibody Biolegend Cat#502505 RRID: AB_315230 APC anti-mouse IFN-g Antibody Biolegend Cat#505809 RRID: AB_315403 FITC anti-mouse IFN-g Antibody Biolegend Cat#505805 RRID: AB_315399 FITC anti-human/mouse Granzyme B Antibody Biolegend Cat#515403 RRID: AB_2114575 APC anti-human/mouse Granzyme B Antibody Biolegend Cat#372203 RRID: AB_2687027 PE anti-human/mouse Granzyme B Antibody Biolegend Cat#372207 RRID: AB_2687031 PE anti-human CD69 Antibody elabscience Cat#E-AB-F1138D Biological samples Prostate cancer patients’ peripheral bloods Nanjing Medical University Affiliated Cancer Hospital N/A Prostate cancer patients’ tumor tissues Nanjing Medical University Affiliated Cancer Hospital N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum (FBS) Gibco Cat#A3160801 b-mercaptoethanol Gibco Cat#21985023 Penicillin-Streptomycin (1003), sterile MCE CatHY-K1006 RBC lysis buffer Sigma Cat#11814389001 Ficoll gradient centrifugation tubes Sigma Cat#26873-85-8 glutamine MAX Gibco Cat#35050087 Recombinant human IL-2 Thermo-Fischer Cat#200–02 Recombinant mouse IL-2 novoprotein Cat#CK24 jetPRIME transfection reagent Polypus Cat#101000027 Proteinase K Roche Cat#3115887001 DAPI Invitrogen Cat#D1306 Cell Lysis Buffer (10X) Cell Signaling Cat#9803 Phosphatase inhibitors Thermo-Fischer Cat#78420 Collagenase IV Gibco Cat#17104019 AZD-5069 MCE Cat#HY-19855 GW6471 MCE Cat#HY-15372 70 mm cell sieve Corning Cat#431751 0.22 mm PVDF filter Millipore Cat#SLGP033RB Amicon Ultra-0.5 Centrifugal Filter Units (100 kDa) Millipore Cat#UFC5100 PVDF Transfer Membrane Millipore Cat#IPVH00010 7-AAD (7-Aminoactinomycin D) Thermo-Fischer Cat# A1310 Critical commercial assays MojoSortTM Human CD8 T cell Isolation Kit Biolegend Cat#480012 MojoSortTM Mouse CD8 T cell Isolation Kit Biolegend Cat#480008 MojoSortTM Mouse CD3 T cell Isolation Kit Biolegend Cat#480024 CFSE Cell Division Tracker Kit Biolegend Cat# 423801 Annexin-V/PI staining kit BD Biosci. .. Cat#556547 Fixation/permeabilization kit BD Biosci.
Lysis:Article Title: Prostate cancer cell-derived exosomal IL-8 fosters immune evasion by disturbing glucolipid metabolism of CD8 + T cell.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FITC anti-human IFN-g Antibody Biolegend Cat#502505 RRID: AB_315230 APC anti-mouse IFN-g Antibody Biolegend Cat#505809 RRID: AB_315403 FITC anti-mouse IFN-g Antibody Biolegend Cat#505805 RRID: AB_315399 FITC anti-human/mouse Granzyme B Antibody Biolegend Cat#515403 RRID: AB_2114575 APC anti-human/mouse Granzyme B Antibody Biolegend Cat#372203 RRID: AB_2687027 PE anti-human/mouse Granzyme B Antibody Biolegend Cat#372207 RRID: AB_2687031 PE anti-human CD69 Antibody elabscience Cat#E-AB-F1138D Biological samples Prostate cancer patients’ peripheral bloods Nanjing Medical University Affiliated Cancer Hospital N/A Prostate cancer patients’ tumor tissues Nanjing Medical University Affiliated Cancer Hospital N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum (FBS) Gibco Cat#A3160801 b-mercaptoethanol Gibco Cat#21985023 Penicillin-Streptomycin (1003), sterile MCE CatHY-K1006 RBC lysis buffer Sigma Cat#11814389001 Ficoll gradient centrifugation tubes Sigma Cat#26873-85-8 glutamine MAX Gibco Cat#35050087 Recombinant human IL-2 Thermo-Fischer Cat#200–02 Recombinant mouse IL-2 novoprotein Cat#CK24 jetPRIME transfection reagent Polypus Cat#101000027 Proteinase K Roche Cat#3115887001 DAPI Invitrogen Cat#D1306 Cell Lysis Buffer (10X) Cell Signaling Cat#9803 Phosphatase inhibitors Thermo-Fischer Cat#78420 Collagenase IV Gibco Cat#17104019 AZD-5069 MCE Cat#HY-19855 GW6471 MCE Cat#HY-15372 70 mm cell sieve Corning Cat#431751 0.22 mm PVDF filter Millipore Cat#SLGP033RB Amicon Ultra-0.5 Centrifugal Filter Units (100 kDa) Millipore Cat#UFC5100 PVDF Transfer Membrane Millipore Cat#IPVH00010 7-AAD (7-Aminoactinomycin D) Thermo-Fischer Cat# A1310 Critical commercial assays MojoSortTM Human CD8 T cell Isolation Kit Biolegend Cat#480012 MojoSortTM Mouse CD8 T cell Isolation Kit Biolegend Cat#480008 MojoSortTM Mouse CD3 T cell Isolation Kit Biolegend Cat#480024 CFSE Cell Division Tracker Kit Biolegend Cat# 423801 Annexin-V/PI staining kit BD Biosci. .. Cat#556547 Fixation/permeabilization kit BD Biosci.
Membrane:Article Title: Prostate cancer cell-derived exosomal IL-8 fosters immune evasion by disturbing glucolipid metabolism of CD8 + T cell.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FITC anti-human IFN-g Antibody Biolegend Cat#502505 RRID: AB_315230 APC anti-mouse IFN-g Antibody Biolegend Cat#505809 RRID: AB_315403 FITC anti-mouse IFN-g Antibody Biolegend Cat#505805 RRID: AB_315399 FITC anti-human/mouse Granzyme B Antibody Biolegend Cat#515403 RRID: AB_2114575 APC anti-human/mouse Granzyme B Antibody Biolegend Cat#372203 RRID: AB_2687027 PE anti-human/mouse Granzyme B Antibody Biolegend Cat#372207 RRID: AB_2687031 PE anti-human CD69 Antibody elabscience Cat#E-AB-F1138D Biological samples Prostate cancer patients’ peripheral bloods Nanjing Medical University Affiliated Cancer Hospital N/A Prostate cancer patients’ tumor tissues Nanjing Medical University Affiliated Cancer Hospital N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum (FBS) Gibco Cat#A3160801 b-mercaptoethanol Gibco Cat#21985023 Penicillin-Streptomycin (1003), sterile MCE CatHY-K1006 RBC lysis buffer Sigma Cat#11814389001 Ficoll gradient centrifugation tubes Sigma Cat#26873-85-8 glutamine MAX Gibco Cat#35050087 Recombinant human IL-2 Thermo-Fischer Cat#200–02 Recombinant mouse IL-2 novoprotein Cat#CK24 jetPRIME transfection reagent Polypus Cat#101000027 Proteinase K Roche Cat#3115887001 DAPI Invitrogen Cat#D1306 Cell Lysis Buffer (10X) Cell Signaling Cat#9803 Phosphatase inhibitors Thermo-Fischer Cat#78420 Collagenase IV Gibco Cat#17104019 AZD-5069 MCE Cat#HY-19855 GW6471 MCE Cat#HY-15372 70 mm cell sieve Corning Cat#431751 0.22 mm PVDF filter Millipore Cat#SLGP033RB Amicon Ultra-0.5 Centrifugal Filter Units (100 kDa) Millipore Cat#UFC5100 PVDF Transfer Membrane Millipore Cat#IPVH00010 7-AAD (7-Aminoactinomycin D) Thermo-Fischer Cat# A1310 Critical commercial assays MojoSortTM Human CD8 T cell Isolation Kit Biolegend Cat#480012 MojoSortTM Mouse CD8 T cell Isolation Kit Biolegend Cat#480008 MojoSortTM Mouse CD3 T cell Isolation Kit Biolegend Cat#480024 CFSE Cell Division Tracker Kit Biolegend Cat# 423801 Annexin-V/PI staining kit BD Biosci. .. Cat#556547 Fixation/permeabilization kit BD Biosci.
Cell Isolation:Article Title: Prostate cancer cell-derived exosomal IL-8 fosters immune evasion by disturbing glucolipid metabolism of CD8 + T cell.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FITC anti-human IFN-g Antibody Biolegend Cat#502505 RRID: AB_315230 APC anti-mouse IFN-g Antibody Biolegend Cat#505809 RRID: AB_315403 FITC anti-mouse IFN-g Antibody Biolegend Cat#505805 RRID: AB_315399 FITC anti-human/mouse Granzyme B Antibody Biolegend Cat#515403 RRID: AB_2114575 APC anti-human/mouse Granzyme B Antibody Biolegend Cat#372203 RRID: AB_2687027 PE anti-human/mouse Granzyme B Antibody Biolegend Cat#372207 RRID: AB_2687031 PE anti-human CD69 Antibody elabscience Cat#E-AB-F1138D Biological samples Prostate cancer patients’ peripheral bloods Nanjing Medical University Affiliated Cancer Hospital N/A Prostate cancer patients’ tumor tissues Nanjing Medical University Affiliated Cancer Hospital N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum (FBS) Gibco Cat#A3160801 b-mercaptoethanol Gibco Cat#21985023 Penicillin-Streptomycin (1003), sterile MCE CatHY-K1006 RBC lysis buffer Sigma Cat#11814389001 Ficoll gradient centrifugation tubes Sigma Cat#26873-85-8 glutamine MAX Gibco Cat#35050087 Recombinant human IL-2 Thermo-Fischer Cat#200–02 Recombinant mouse IL-2 novoprotein Cat#CK24 jetPRIME transfection reagent Polypus Cat#101000027 Proteinase K Roche Cat#3115887001 DAPI Invitrogen Cat#D1306 Cell Lysis Buffer (10X) Cell Signaling Cat#9803 Phosphatase inhibitors Thermo-Fischer Cat#78420 Collagenase IV Gibco Cat#17104019 AZD-5069 MCE Cat#HY-19855 GW6471 MCE Cat#HY-15372 70 mm cell sieve Corning Cat#431751 0.22 mm PVDF filter Millipore Cat#SLGP033RB Amicon Ultra-0.5 Centrifugal Filter Units (100 kDa) Millipore Cat#UFC5100 PVDF Transfer Membrane Millipore Cat#IPVH00010 7-AAD (7-Aminoactinomycin D) Thermo-Fischer Cat# A1310 Critical commercial assays MojoSortTM Human CD8 T cell Isolation Kit Biolegend Cat#480012 MojoSortTM Mouse CD8 T cell Isolation Kit Biolegend Cat#480008 MojoSortTM Mouse CD3 T cell Isolation Kit Biolegend Cat#480024 CFSE Cell Division Tracker Kit Biolegend Cat# 423801 Annexin-V/PI staining kit BD Biosci. .. Cat#556547 Fixation/permeabilization kit BD Biosci.
Staining:Article Title: Prostate cancer cell-derived exosomal IL-8 fosters immune evasion by disturbing glucolipid metabolism of CD8 + T cell.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER FITC anti-human IFN-g Antibody Biolegend Cat#502505 RRID: AB_315230 APC anti-mouse IFN-g Antibody Biolegend Cat#505809 RRID: AB_315403 FITC anti-mouse IFN-g Antibody Biolegend Cat#505805 RRID: AB_315399 FITC anti-human/mouse Granzyme B Antibody Biolegend Cat#515403 RRID: AB_2114575 APC anti-human/mouse Granzyme B Antibody Biolegend Cat#372203 RRID: AB_2687027 PE anti-human/mouse Granzyme B Antibody Biolegend Cat#372207 RRID: AB_2687031 PE anti-human CD69 Antibody elabscience Cat#E-AB-F1138D Biological samples Prostate cancer patients’ peripheral bloods Nanjing Medical University Affiliated Cancer Hospital N/A Prostate cancer patients’ tumor tissues Nanjing Medical University Affiliated Cancer Hospital N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum (FBS) Gibco Cat#A3160801 b-mercaptoethanol Gibco Cat#21985023 Penicillin-Streptomycin (1003), sterile MCE CatHY-K1006 RBC lysis buffer Sigma Cat#11814389001 Ficoll gradient centrifugation tubes Sigma Cat#26873-85-8 glutamine MAX Gibco Cat#35050087 Recombinant human IL-2 Thermo-Fischer Cat#200–02 Recombinant mouse IL-2 novoprotein Cat#CK24 jetPRIME transfection reagent Polypus Cat#101000027 Proteinase K Roche Cat#3115887001 DAPI Invitrogen Cat#D1306 Cell Lysis Buffer (10X) Cell Signaling Cat#9803 Phosphatase inhibitors Thermo-Fischer Cat#78420 Collagenase IV Gibco Cat#17104019 AZD-5069 MCE Cat#HY-19855 GW6471 MCE Cat#HY-15372 70 mm cell sieve Corning Cat#431751 0.22 mm PVDF filter Millipore Cat#SLGP033RB Amicon Ultra-0.5 Centrifugal Filter Units (100 kDa) Millipore Cat#UFC5100 PVDF Transfer Membrane Millipore Cat#IPVH00010 7-AAD (7-Aminoactinomycin D) Thermo-Fischer Cat# A1310 Critical commercial assays MojoSortTM Human CD8 T cell Isolation Kit Biolegend Cat#480012 MojoSortTM Mouse CD8 T cell Isolation Kit Biolegend Cat#480008 MojoSortTM Mouse CD3 T cell Isolation Kit Biolegend Cat#480024 CFSE Cell Division Tracker Kit Biolegend Cat# 423801 Annexin-V/PI staining kit BD Biosci. .. Cat#556547 Fixation/permeabilization kit BD Biosci.
|
MedChemExpress is a verified supplier 
MedChemExpress manufactures this product 